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trap solution buffer  (Merck & Co)

 
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    Structured Review

    Merck & Co trap solution buffer
    Trap Solution Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+buffer+solution/buffer+solution/pmc12445851-53-46-52
    Average 86 stars, based on 1 article reviews
    trap solution buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: G protein-coupled receptor 35 stimulation reduces osteoclast activity in primary human bone cells
    Article Snippet: Sample images were taken using Olympus cellSens software (Olympus). .. On day 10 of differentiation, all cells were collected using Accutase and cell scraping, then seeded in 96-well plates and exposed to agonists and/or antagonists for 72 h. Conditioned media was collected and transferred in 10 μL duplicates into a 96-well clear plate, then 90 μL TRAP solution buffer (1 M acetate (Merck), 0.5% Triton X-100 (Sigma-Aldrich), 1 M NaCl (Sigma-Aldrich), 10 mM EDTA (Fisher Scientific), 50 mM L-Ascorbic acid (Merck), 0.2 M disodium tartrate (Sigma-Aldrich), 82 mM 4-nitrolphenylphosphate (Sigma-Aldrich)) added to each well, prior to incubation in the dark for 30 min at 37 °C. .. The reaction was stopped by adding 0.3 M NaOH (Honeywell, SLS, Hessle, United Kingdom) and absorbance was measured at 405 nm on a SpectraMax ABS (Molecular Devices) or GloMax plate reader.

    Article Title: G protein-coupled receptor 35 (GPR35) stimulation reduces osteoclast activity in primary human bone cells
    Article Snippet: Sample images were taken using Olympus cellSens software (Olympus). .. Tartrate-resistant acid phosphatase 5b activity assays On day 10 of differentiation, all cells were collected using Accutase and cell scraping, then seeded in 96-well plates and exposed to agonists and/or antagonists for 72 h. Conditioned media was collected and transferred in 10 μL duplicates into a 96-well clear plate, then 90 μL TRAP solution buffer (1 M acetate (Merck), 0.5% Triton X-100 (Sigma-Aldrich), 1 M NaCl (Sigma-Aldrich), 10 mM EDTA (Fisher Scientific), 50 mM L-Ascorbic acid (Merck), 0.2 M disodium tartrate (Sigma-Aldrich), 82 mM 4-nitrolphenylphosphate (SigmaAldrich)) added to each well, prior to incubation in the dark for 30 min at 37 ◦C. .. The reaction was stopped by adding 0.3 M NaOH (Honeywell, SLS, Hessle, United Kingdom) and absorbance was measured at 405 nm on a SpectraMax ABS (Molecular Devices) or GloMax plate reader.

    Activity Assay:

    Article Title: G protein-coupled receptor 35 (GPR35) stimulation reduces osteoclast activity in primary human bone cells
    Article Snippet: Sample images were taken using Olympus cellSens software (Olympus). .. Tartrate-resistant acid phosphatase 5b activity assays On day 10 of differentiation, all cells were collected using Accutase and cell scraping, then seeded in 96-well plates and exposed to agonists and/or antagonists for 72 h. Conditioned media was collected and transferred in 10 μL duplicates into a 96-well clear plate, then 90 μL TRAP solution buffer (1 M acetate (Merck), 0.5% Triton X-100 (Sigma-Aldrich), 1 M NaCl (Sigma-Aldrich), 10 mM EDTA (Fisher Scientific), 50 mM L-Ascorbic acid (Merck), 0.2 M disodium tartrate (Sigma-Aldrich), 82 mM 4-nitrolphenylphosphate (SigmaAldrich)) added to each well, prior to incubation in the dark for 30 min at 37 ◦C. .. The reaction was stopped by adding 0.3 M NaOH (Honeywell, SLS, Hessle, United Kingdom) and absorbance was measured at 405 nm on a SpectraMax ABS (Molecular Devices) or GloMax plate reader.



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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Image Search Results


    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Journal: Frontiers in Immunology

    Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure

    doi: 10.3389/fimmu.2022.817281

    Figure Lengend Snippet: Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Article Snippet: The staining solution consists of TRAP buffer (0.1 M acetate buffer: 35.2 ml 0.2 M Sodium Acetate solution, 14.8 ml 0.2 M acetic acid, 50 ml; Millipore, Burlington, USA) mixed with 0.3 M Sodium Tartrate (pH 5.5), 10 mg/ml Naphthol AS-MX phosphate, Triton X-100, 0.3 mg/ml Fast Red Violet LB Salt (all from Sigma Aldrich Chemie GmbH).

    Techniques: Activity Assay, Staining, Microscopy